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low-binding microtiter plates corning 3881  (Corning Life Sciences)

 
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    Corning Life Sciences low-binding microtiter plates corning 3881
    Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the <t>microtiter</t> plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .
    Low Binding Microtiter Plates Corning 3881, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-binding microtiter plates corning 3881/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    low-binding microtiter plates corning 3881 - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor"

    Article Title: Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor

    Journal: ACS Catalysis

    doi: 10.1021/acscatal.4c00230

    Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the microtiter plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .
    Figure Legend Snippet: Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the microtiter plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .

    Techniques Used: Expressing, Emulsion, Lysis, Concentration Assay, Fluorescence, Selection, Double Emulsion, FACS, Flow Cytometry



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    low-binding microtiter plates corning 3881  (Corning Life Sciences)
    90
    Corning Life Sciences low-binding microtiter plates corning 3881
    Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the <t>microtiter</t> plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .
    Low Binding Microtiter Plates Corning 3881, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-binding microtiter plates corning 3881/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    low-binding microtiter plates corning 3881 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    96-well black half-area, low binding, transparent bottom microtiter plates corning #3881  (Corning Life Sciences)
    90
    Corning Life Sciences 96-well black half-area, low binding, transparent bottom microtiter plates corning #3881
    Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the <t>microtiter</t> plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .
    96 Well Black Half Area, Low Binding, Transparent Bottom Microtiter Plates Corning #3881, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96-well black half-area, low binding, transparent bottom microtiter plates corning #3881/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    96-well black half-area, low binding, transparent bottom microtiter plates corning #3881 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the microtiter plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .

    Journal: ACS Catalysis

    Article Title: Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor

    doi: 10.1021/acscatal.4c00230

    Figure Lengend Snippet: Overview of the screening approach. (a) TrpB-expressing cells are encapsulated in single emulsion droplets with the aptamer sensor, the substrates (Ser, indole), and lysis reagents. (b) One —Lysis reagents will release TrpB in each droplet where a cell is present. Two —TrpB catalysis of Ser and indole to produce Trp. Three —The fluorescent aptamer is initially self-quenched, but once Trp is bound in favor of the quenching complementary strand, the sensor lights up and becomes fluorescent. The Trp concentration measured as a fluorescence signal is a function of the catalytic efficiency, stability, and expression strength of TrpB variants, and screening and selection can be carried out accordingly. (c) Droplets are encapsulated again into double emulsion droplets so that they are compatible with fluorescence-activated cell sorting on a commercial flow cytometer. (d) Genotype from the pool of highly fluorescent droplets is recovered, after which the enriched pool of active variants is rescreened in the microtiter plate-based format for single variants of interest. The chip design is shown in Supplementary Figure 1 .

    Article Snippet: The aptamer sensor was combined 1:1 either with purified chemicals or with TrpB reaction mixtures in a final volume of 80 μL in low-binding microtiter plates (Corning, 3881).

    Techniques: Expressing, Emulsion, Lysis, Concentration Assay, Fluorescence, Selection, Double Emulsion, FACS, Flow Cytometry